EIF5A2 Protein (AA 1-153) (His tag)

Catalog No. ABIN1692563

Recombinant EIF5A2 protein expressed in Escherichia coli (E. coli).

Quick Overview for EIF5A2 Protein (AA 1-153) (His tag) (ABIN1692563)

Target: EIF5A2 (Eukaryotic Translation Initiation Factor 5A2 (EIF5A2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-153

Purification tag / Conjugate: This EIF5A2 protein is labelled with His tag.

Purpose: Recombinant Human Eukaryotic Translation Initiation Factor 5A-2/EIF5A2 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MADEIDFTTG DAGASSTYPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDYQ LICIQDGYLS LLTETGEVRE DLKLPEGELG KEIEGKYNAG EDVQVSVMCA MSEEYAVAIK PCK

Characteristics:

Recombinant Human Eukaryotic Translation Initiation Factor 5A-2/EIF5A2 (N-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: EIF5A2 (Eukaryotic Translation Initiation Factor 5A2 (EIF5A2))

Alternative Name: Eukaryotic Translation Initiation Factor 5A-2/EIF5A2

Background: Recombinant Human Eukaryotic translation initiation factor 5A-2/EIF5A2 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys153) of Human EIF5A2 fused with a 6His tag at the N-terminus.

Molecular Weight: 18.9 kDa

UniProt: Q9GZV4