Ephrin B1 Protein (EFNB1) (AA 28-232) (His tag)

Catalog No. ABIN1880659

Recombinant Ephrin B1 protein expressed in Human Cells.

Quick Overview for Ephrin B1 Protein (EFNB1) (AA 28-232) (His tag) (ABIN1880659)

Target: Ephrin B1 (EFNB1)

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 28-232

Purification tag / Conjugate: This Ephrin B1 protein is labelled with His tag.

Purpose: Recombinant Human Ephrin-B1/EFNB1 (C-6His)

Sequence: LAKNLEPVSW SSLNPKFLSG KGLVIYPKIG DKLDIICPRA EAGRPYEYYK LYLVRPEQAA ACSTVLDPNV LVTCNRPEQE IRFTIKFQEF SPNYMGLEFK KHHDYYITST SNGSLEGLEN REGGVCRTRT MKIIMKVGQD PNAVTPEQLT TSRPSKEADN TVKMATQAPG SRGSLGDSDG KHETVNQEEK SGPGASGGSS GDPDGVDHHH HHH

Characteristics: Recombinant Human Ephrin-B1/EFNB1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu28-Gly232) of Human EFNB1 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Ephrin B1 (EFNB1)

Alternative Name: Ephrin-B1/EFNB1

Sub Type: Fusionprotein

Background: Ephrin-B1, also named EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, is a single-pass type I membrane protein. It contains 1 ephrin RBD (ephrin receptor-binding) domain and belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4. The extracellular domains of human and mouse ephrin-B1 share 94% amino acid identity.
Alternative Names: Ephrin-B1, also named EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, is a single-pass type I membrane protein.

Molecular Weight: 23.4 kDa

UniProt: P98172

Pathways: RTK Signaling