Collagen alpha-1 (AA 155-1219) protein (His tag)

Catalog No. ABIN1945409

This Recombinant Collagen alpha-1 protein is expressed in Human Cells.

Quick Overview for Collagen alpha-1 (AA 155-1219) protein (His tag) (ABIN1945409)

Target: Collagen alpha-1

Protein Type: Recombinant

Origin: Mouse

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 155-1219

Purification tag / Conjugate: His tag

Purpose: Recombinant Mouse Collagen α-1(III) Chain/COL3A1 (C-6His)

Sequence: QFDSYDVKSG VGGMGGYPGP AGPPGPPGPP GSSGHPGSPG SPGYQGPPGE PGQAGPAGPP GPPGALGPAG PAGKDGESGR PGRPGERGLP GPPGIKGPAG MPGFPGMKGH RGFDGRNGEK GETGAPGLKG ENGLPGDNGA PGPMGPRGAP GERGRPGLPG AAGARGNDGA RGSDGQPGPP GPPGTAGFPG SPGAKGEVGP AGSPGSNGSP GQRGEPGPQG HAGAQGPPGP PGNNGSPGGK GEMGPAGIPG APGLIGARGP PGPAGTNGIP GTRGPSGEPG KNGAKGEPGA RGERGEAGSP GIPGPKGEDG KDGSPGEPGA NGLPGAAGER GPSGFRGPAG PNGIPGEKGP PGERGGPGPA GPRGVAGEPG RDGTPGGPGI RGMPGSPGGP GNDGKPGPPG SQGESGRPGP PGPSGPRGQP GVMGFPGPKG NDGAPGKNGE RGGPGGPGLP GPAGKNGETG PQGPPGPTGP AGDKGDSGPP GPQGLQGIPG TGGPPGENGK PGEPGPKGEV GAPGAPGGKG DSGAPGERGP PGTAGIPGAR GGAGPPGPEG GKGPAGPPGP PGASGSPGLQ GMPGERGGPG SPGPKGEKGE PGGAGADGVP GKDGPRGPAG PIGPPGPAGQ PGDKGEGGSP GLPGIAGPRG GPGERGEHGP PGPAGFPGAP GQNGEPGAKG ERGAPGEKGE GGPPGPAGPT GSSGPAGPPG PQGVKGERGS PGGPGTAGFP GGRGLPGPPG NNGNPGPPGP SGAPGKDGPP GPAGNSGSPG NPGIAGPKGD AGQPGEKGPP GAQGPPGSPG PLGIAGLTGA RGLAGPPGMP GPRGSPGPQG IKGESGKPGA SGHNGERGPP GPQGLPGQPG TAGEPGRDGN PGSDGQPGRD GSPGGKGDRG ENGSPGAPGA PGHPGPPGPV GPSGKSGDRG ETGPAGPSGA PGPAGARGAP GPQGPRGDKG ETGERGSNGI KGHRGFPGNP GPPGSPGAAG HQGAIGSPGP AGPRGPVGPH GPPGKDGTSG HPGPIGPPGP RGNRGERGSE GSPGHPGQPG PPGPPGAPGP CCGGGAAAIA GVGGEKSGGF SPYYGVDHHH HHH

Characteristics: Recombinant Mouse Collagen alpha-1(III) chain/COL3A1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (AA 155-1219) of Mouse COL3A1 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM HAc-NaAc,150 mM NaCl, pH 4.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Collagen alpha-1

Background: Collagen alpha-1(III) chain（Col3a1） is a secreted protein and belongs to the fibrillar collagen family.It contains 1 fibrillar collagen NC1 domain and 1 VWFC domain. Collagen alpha-1(III) chain is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I collagen. The COL3A1 gene produces the components of type III collagen, called pro-alpha1(III) chains. Three copies of this chain combine to make a molecule of type III procollagen. These triple-stranded, rope-like procollagen molecules must be processed by enzymes outside the cell to remove extra protein segments from their ends. Once these molecules are processed, the collagen molecules arrange themselves into long, thin fibrils. Within these fibrils, the individual collagen molecules are cross-linked to one another. These cross-links result in the formation of very strong mature type III collagen fibrils, which are found in the spaces around cells.

Molecular Weight: 96.6 kDa

UniProt: P08121