Collagen alpha-1 (AA 24-328) protein (His tag)

Catalog No. ABIN1945429

Recombinant Collagen alpha-1 protein expressed in Human Cells.

Quick Overview for Collagen alpha-1 (AA 24-328) protein (His tag) (ABIN1945429)

Target: Collagen alpha-1

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 24-328

Purification tag / Conjugate: His tag

Purpose: Recombinant Human Collagen α-1(IX) Chain/COL9A1 (C-6His)

Sequence: AVKRRPRFPV NSNSNGGNEL CPKIRIGQDD LPGFDLISQF QVDKAASRRA IQRVVGSATL QVAYKLGNNV DFRIPTRNLY PSGLPEEYSF LTTFRMTGST LKKNWNIWQI QDSSGKEQVG IKINGQTQSV VFSYKGLDGS LQTAAFSNLS SLFDSQWHKI MIGVERSSAT LFVDCNRIES LPIKPRGPID IDGFAVLGKL ADNPQVSVPF ELQWMLIHCD PLRPRRETCH ELPARITPSQ TTDERGPPGE QGPPGPPGPP GVPGIDGIDG DRGPKGPPGP PGPAGEPGKP GAPGKPGTPG ADTSPVDHHH HHH

Characteristics: Recombinant Human Collagen alpha-1(IX) chain/ COL9A1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (AA 24-328) of Human COL9A1 fused with a polyhistidine tag at the C-termin

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Collagen alpha-1

Background: COL9A1, which is short for Collagen alpha-1(IX) chain, is a 921 aa. protein. It is a secreted protein, and exists in extracellular space and extracellular matrix. This protein is a heterotrimer of an alpha 1(IX), an alpha 2(IX) and an alpha 3(IX) chain.Each subunit is composed of three triple-helical domains interspersed with non-collagenous domains. The globular domain at the N-terminus of type IX collagen molecules represents the NC4 domain which may participate in electrostatic interactions with polyanionic glycosaminoglycans in cartilage. It ia a structural component of hyaline cartilage and vitreous of the eye.

Molecular Weight: 33.8 kDa