CNTF Protein (AA 2-200)

Catalog No. ABIN2017715

Recombinant CNTF protein expressed in Escherichia coli (E. coli).

Quick Overview for CNTF Protein (AA 2-200) (ABIN2017715)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 2-200

Characteristics: ED50 < 200 ng/mL, measured cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 5x10^3 units/mg.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Ciliary Neurotrophic Factor (rhCNTF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhCNTF remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Background: Ciliary Neurotrophic Factor (CNTF) is a cytokine belonging to the Interleukin 6 (IL-6) family, which also includes IL-6, Oncostatin M, Leukemia Inhibitory Factor (LIF), and Cardiotrophin-1. Structurally, CNTF resembles a four-helix bundle composition, similar to the other members of the IL-6 family. The receptor for CNTF is composed of three parts: a gp130-like subunit common in the IL-6 receptor family, a LIF Receptor beta subunit, and a CNTF specific alpha receptor subunit. Upon binding to the CNTF, the beta subunit of the CNTF receptor will undergo tyrosine phosphorylation, and activate the intracellular JAK/STAT pathway. The main function of CNTF in vivo is to promote the differentiation and survival of a variety of neurons and glial cells, including sympathetic precursor cells and spinal motor neurons.Recombinant human Ciliary Neurotrophic Factor (rhCNTF) produced in E. coli is a single non-glycosylated polypeptide chain containing 199 amino acids. A fully biologically active molecule, rhCNTF has a molecular mass of 22.8 kDa analyzed by reducing SDS-PAGE.
Synonyms: Ciliary Neurotrophic Factor

Molecular Weight: 22.8 kDa, observed by reducing SDS-PAGE.

UniProt: P26441

Pathways: JAK-STAT Signaling