TMPRSS15 Protein

Catalog No. ABIN2017742

This Recombinant TMPRSS15 protein is produced in Yeast (Pichia pastoris).

Quick Overview for TMPRSS15 Protein (ABIN2017742)

Target: TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

Protein Type: Recombinant

Origin: Cow

Source: Yeast (Pichia pastoris)

Purity: > 95 % by SDS-PAGE analyses.

Product Details

Characteristics: 5 IU/μl
Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 22 °C in a buffer containing 25 mM Tris-HCl, pH 8.0.

Sterility: Sterile

Endotoxin Level: < 1.0 EU/μg, determined by LAL method.

Components: 100 IU (or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
100 μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl2) Storage: Store kit components at -20 °C.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Sterile liquid solution contians 20 mM Tris, 200 mM NaCl, 2 mM CaCl2, 50 % glycerol, pH 7.4.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20 °C from date of receipt. It will remain stable at 37 °C for one week without losing any activity.

Expiry Date: 12 months

Target Details

Target: TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

Alternative Name: Enterokinase(EK)

Background: Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques.
Synonyms: Enteropeptidase, ENTK, PRSS7

Molecular Weight: Theoretical MW: 22.7 kDa.
Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.