FGF18 Protein (AA 28-199)

Catalog No. ABIN2017814

This Recombinant FGF18 protein is expressed in Escherichia coli (E. coli).

Quick Overview for FGF18 Protein (AA 28-199) (ABIN2017814)

Target: FGF18 (Fibroblast Growth Factor 18 (FGF18))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 28-199

Characteristics: ED50 < 0.5 xg/mL, measured by a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2x 10^3 units/mg.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant rat Fibroblast Growth Factor 18 (rrFGF-18) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrFGF-18 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF18 (Fibroblast Growth Factor 18 (FGF18))

Alternative Name: Fibroblast Growth Factor-18 (FGF-18)

Background: Fibroblast Growth Factor 18 (FGF-18) is a pleiotropic cytokine belonging to the heparin-binding FGF family, which has 23 different members. Structurally, FGF-18 is closely related to FGF-8 and FGF-17. Like other FGFs, FGF-18 can bind to different FGF receptors in vivo. FGF-18 is expressed in various tissues and has multiple functions: during long bone growth, FGF-18 is expressed in perichondrium and developing joints, and regulates bone formation by inhibiting chondrocyte proliferation and differentiation, FGF-18 knock-out mice survive embryonic development, but exhibit skeletal abnormalities and die in the early neonatal period. FGF-18 also induces ectopic cartilage formation in the lung, and alters the morphology of the pulmonary mesenchyma. Recombinant rat Fibroblast Growth Factor 18 (rrFGF-18) produced in E. coli is a single non-glycosylated polypeptide chain containing of 172 amino acids. A fully biologically active molecule, rrFGF-18 has a molecular mass of 20.1 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: Fibroblast growth factor 18, FGF-18, zFGF5, Fgf18, D130055P09Rik

Molecular Weight: 20.1 kDa, observed by non-reducing SDS-PAGE.

UniProt: O88182

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway