Heregulin1-Beta1(HRG1-Beta1) (AA 177-241), (Isoform 6) (Active) Protein

Catalog No. ABIN2017957

Recombinant protein expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for Heregulin1-Beta1(HRG1-Beta1) (AA 177-241), (Isoform 6) (Active) Protein (ABIN2017957)

Target: Heregulin1-Beta1(HRG1-Beta1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE analysis.

Product Details

Protein Characteristics: Isoform 6, AA 177-241

Characteristics: ED50 < 1 ng/mL, measured by a cell proliferation assay using MCF-7 cells, corresponding to a specific activity of > 1x 10^6 units/mg.
AA 177-241 (isoform 6), expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Heregulin1-Beta1(HRG1-Beta1) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhHRG1-Beta1 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

References

Hedayatizadeh-Omran, Valadan, Rafiei, Tehrani, Alizadeh-Navaei: "VERO Stable Cell Lines Expressing Full-Length Human Epidermal Growth Factor Receptors 2 and 3: Platforms for Subtractive Phage Display." in: DNA and cell biology, Vol. 34, Issue 9, pp. 573-8, (2015) (PubMed).

Target Details

Target: Heregulin1-Beta1(HRG1-Beta1)

Background: Heregulin1-Beta1(HRG1-Beta1) is one of the isoforms encoded by Neuregulin (NRG) genes. NRGs are synthesized as large transmembrane precursor proteins, and the NRG family has 4 members and 26 isoforms. These isoforms provide large diversities, including different tissue distribution, variable potencies, and different biological functions. HRG1-beta1 belongs to Type I HRG1, and is expressed in neural tissue, respiratory epithelia, and heart. In vivo, HRG1 binds and activates both ErbB3 and ErbB4, the transmembrane receptor tyrosine kinase, and is involved in the proliferation, differentiation, and survival of cells. Aberrantly produced HRG1 could be used in the constitute activation of the ErbB receptors, therefore, the upregulation of HRG1 contributes to the development of tumors, including breast cancer.Recombinant human Heregulin1-Beta1(HRG1-Beta1) produced in E. coli is a single non-glycosylated polypeptide chain containing 66 amino acids. A fully biologically active molecule, rh HRG1-Beta1 has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE.
Synonyms: Neuregulin1 (NRG1-beta 1)

Molecular Weight: 7.6 kDa, observed by reducing SDS-PAGE.

UniProt: Q02297