IGF2 Protein (AA 25-91, Isoform 1)

Catalog No. ABIN2017988

This Recombinant IGF2 protein is produced in Escherichia coli (E. coli).

Quick Overview for IGF2 Protein (AA 25-91, Isoform 1) (ABIN2017988)

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 25-91, Isoform 1

Characteristics: ED50 < 20 ng/mL, measured by a cell proliferation assay using FDCP-1 cells, corresponding to a specific activity of > 5x10^4 units/mg.
AA 25-91 (isoform I), expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 25 mM Tris, pH 8.0.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Insulin-like Growth Factor II (rhIGF-II) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIGF-II remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Alternative Name: Insulin-Like Growth Factor II (IGF-II)

Background: Insulin-like Growth Factor II (IGF-II) is a single chain 7 kDa polypeptide, and shares a high degree of homology with insulin. During circulation in vivo, IGF-II is complexed to high affinity binding proteins, IGF Binding Proteins (IGFBP), which act as circulating reservoirs, transport IGF-II, and prolong the half life of IGF-II. The receptors of IGF-II (IGFRs) are transmembrane tyrosine receptors, and are heterotetrameric consisting of two alpha-subunits and two beta-subunits. IGFRs execute their role via intracellullar signaling molecules, such as IRS, shc, and PI3K. The functions of IGF-II include promoting cell survival, growth, proliferation, differentiation and motility. In particular, IGF-II promotes proliferation, inhibits death, and stimulates transformation in breast cancer cells.Recombinant human Insulin-like Growth Factor II (rhIGF-II) produced in E. coli is a single non-glycosylated polypeptide chain containing 68 amino acids. A fully biologically active molecule, rhIGF-II has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE.
Synonyms: Insulin-like Growth Factor-II, Somatamedin A

Molecular Weight: 7.6 kDa, observed by reducing SDS-PAGE.

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation