IGFBP4 Protein (AA 22-258) (His tag)

Catalog No. ABIN2017993

This Recombinant IGFBP4 protein is produced in HEK-293T Cells.

Quick Overview for IGFBP4 Protein (AA 22-258) (His tag) (ABIN2017993)

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293T Cells

Purity: > 95 % as analyzed by SDS-PAGE.

Product Details

Protein Characteristics: AA 22-258

Purification tag / Conjugate: This IGFBP4 protein is labelled with His tag.

Characteristics: ED50 < 50 ng/mL, measured in a bioassay using FDC-P1 cells in the presence of 15 ng/mL human IGF-II.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human IGF-BP-4 remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, human IGF-BP-4, His should be stable up to 1 week at 4 °C or up to 2 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Alternative Name: Insulin-Like Growth Factor-Binding Protein 4 (IGF-BP-4)

Background: Insulin-like growth factor-binding protein 4 (IGF-BP-4), also known as IBP-4, is a secreted glycoprotein belonging to the IGFBP family. IGF-BP-4 is produced by osteoblasts, epidermis, ovarian follicles and other tissues. It binds both insulin-like growth factor (IGF) I and II, and it circulates in the plasma in both glycosylated and non-glycosylated forms. IGF-BP-4 prolongs the half-life of the IGFs and has been shown to inhibit or stimulate the growth-promoting effects of the IGFs. Pregnancy Associated Plasma Protein A (PAPP-A) proteolytically cleaves IGF-BP-4 and reduces its affinity to bind IGFs, and thus serves as an important regulator of IGF-BP-4 function.
Synonyms: Insulin-like Growth Factor-Binding Protein 4, IBP-4, HT29-IGF-BP, colon cancer cell growth inhibitor

Molecular Weight: 30-35 kDa, observed by reducing SDS-PAGE.

UniProt: P22692

Pathways: WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process