Interferon gamma Protein (IFNG) (AA 23-155)

Catalog No. ABIN2018008

This Recombinant Interferon gamma protein is produced in Escherichia coli (E. coli).

Quick Overview for Interferon gamma Protein (IFNG) (AA 23-155) (ABIN2018008)

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as analyzed by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 23-155

Characteristics: ED50<0.15 ng/mL, measured by cytotoxicity assay using WEHI-279 cells.
AA 23-155, expressed with an N-terminal Met.

Endotoxin Level: < 1 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant mouse IFN gamma (rmIFN-γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIFN-γ should be stable up to 1week at 4°C or up to 2 months at -20°C.

Expiry Date: 6 months

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: Interferon-gamma (IFN-Gamma)

Background: Sharing 41 % sequence identity with human Interferon gamma (hIFN--γ), mouse IFN gamma (mIFN--γ) is a macrophage-activating factor.The active form of IFN--γ is an antiparallel dimer that sets off IFN--γ/JAK/STAT pathway. IFN--γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity.While IFN--γ-induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN--γ is also implicated in resistance to NK cell and CTL responses and in immune escape in avariety of cancers. Recombinant mouse IFN gamma (rmIFN-γ) produced in E. coli is a non-glycosylated polypeptide chain of 134 amino acids. A fully biologically active molecule, rmIFN--γ has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Synonyms: Type II interferon, T cell interferon, MAF, IFNG, IFG, IFI

Molecular Weight: 15 kDa, observed by reducing SDS-PAGE.

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy