IL-3 Protein (AA 20-152)

Catalog No. ABIN2018099

Recombinant IL-3 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-3 Protein (AA 20-152) (ABIN2018099)

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 20-152

Characteristics: ED50 < 0.5 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 2 x 10^7 units/mg.
AA 20-152, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Interleukin-3 (rhIL-3) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIL-3 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: Interleukin-3 (IL-3)

Background: Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific alpha chain and a common beta chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family. Recombinant human Interleukin-3 (rhIL-3) produced in E. coli is a single non-glycosylated polypeptide chain containing of 134 amino acids. A fully biologically active molecule, rhIL-3 has a molecular mass of 15.2 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor

Molecular Weight: 15.2 kDa, observed by non-reducing SDS-PAGE.

UniProt: P08700

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy