KIT Ligand Protein (KITLG) (AA 26-189)
Quick Overview for KIT Ligand Protein (KITLG) (AA 26-189) (ABIN2018380)
Target
See all KIT Ligand (KITLG) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 26-189
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Characteristics
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ED50 < 10.0 ng/mL, measured by a cell proliferation assay using human TF-1 cells, corresponding to a specific activity of > 1.0x 10^5 units/mg.
AA 26-189, expressed with an N-terminal Met. -
Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
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KIT Ligand (KITLG) (Active) protein
KITLG Origin: Human Host: Yeast (Pichia pastoris) > 95 % by SDS-PAGE Active
KIT Ligand (KITLG) (AA 26-214) protein (Fc Tag)KITLG Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 26-189) (Active) proteinKITLG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS, AcA Active
KIT Ligand (KITLG) (AA 26-189) protein (His tag)KITLG Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-190) protein (His tag)KITLG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) (AA 26-189) protein (His-IF2DI Tag)KITLG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
KIT Ligand (KITLG) protein (His tag)KITLG Origin: Rat Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
KIT Ligand (KITLG) (AA 1-245) protein (GST tag)KITLG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstituted in ddH2O at 100 μg/mL.
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Buffer
- Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.
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Storage
- -80 °C
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Storage Comment
- Lyophilized recombinant mouse Stem Cell Factor (rmSCF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmSCF should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
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Expiry Date
- 6 months
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- KIT Ligand (KITLG)
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Alternative Name
- Stem Cell Factor (SCF)
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Background
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Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant mouse Stem Cell Factor (rmSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rmSCF has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor -
Molecular Weight
- 18.4kDa, observed by reducing SDS-PAGE.
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UniProt
- P20826
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Target
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