VEGF-A121 (AA 28-147) (Active) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 95 % as analyzed by SDS-PAGE and HPLC.
Active
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- Target
- VEGF-A121
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 28-147
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Characteristics
-
ED50 < 5 ng/mL, measured by a cell proliferation assay using HUVEC Cells, corresponding to a specific activity of > 2x 10^5 units/mg.
AA 28-147, expressed with an N-terminal Met. - Purity
- > 95 % as analyzed by SDS-PAGE and HPLC.
- Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
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- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O or PBS at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant human VEGF-A121 (rhVEGF-A121) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhVEGF-A121 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
- Expiry Date
- 6 months
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- Target
- VEGF-A121
- Background
-
VEGF-A121 is one of five isoforms (121, 145, 165, 189, and 206) of VEGF protein, a cytokine belonging to the Platelet Differentiation Growth Factor (PDGF) family, and existing as a disulfide-linked homodimeric glycoprotein. In contrast to the longer isoforms, VEGF-A121 is more freely diffusible, and cannot bind to heparin. In vivo, VEGF is expressed predominantly in lung, heart, kidney, and adrenal glands, and the expression of VEGF is up-regulated by a number of growth factors, including PDGF, Fibroblast Growth Factor (FGF), Epidermal Growth Factor (EGF), and Tumor Necrosis Factor (TNF). VEGF signals via binding to two tyrosine kinase receptors: the Fms-like tyrosine kinase 1 (Flt-1) and the kinase domain receptor (KDR). VEGF is a specific mitogen and survival factor, contributing to abnormal angiogenesis and cancer development. Recombinant human VEGF-A121 (rhVEGF-A121) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 121 amino acids each. A fully biologically active molecule, rhVEGF-A121 has a molecular mass of 28.2 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: Vascular Endothelial Growth Factor121, VPF - Molecular Weight
- 28.2 kDa, observed by non-reducing SDS-PAGE.
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