Fibronectin Protein (AA 1270-1546, AA 1721-2016)

Catalog No. ABIN2115447

This Recombinant Fibronectin protein is produced in Escherichia coli (E. coli).

Quick Overview for Fibronectin Protein (AA 1270-1546, AA 1721-2016) (ABIN2115447)

Target: Fibronectin

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1270-1546, AA 1721-2016

Purpose: Recombinant Human Fibronectin Fragment/RetroNectin/NovoNectin

Sequence: MPTDLRFTNI GPDTMRVTWA PPPSIDLTNF LVRYSPVKNE EDVAELSISP SDNAVVLTNL LPGTEYVVSV SSVYEQHEST PLRGRQKTGL DSPTGIDFSD ITANSFTVHW IAPRATITGY RIRHHPEHFS GRPREDRVPH SRNSITLTNL TPGTEYVVSI VALNGREESP LLIGQQSTVS DVPRDLEVVA ATPTSLLISW DAPAVTVRYY RITYGETGGN SPVQEFTVPG SKSTATISGL KPGVDYTITV YAVTGRGDSP ASSKPISINY RTEIDKPSMA IPAPTDLKFT QVTPTSLSAQ WTPPNVQLTG YRVRVTPKEK TGPMKEINLA PDSSSVVVSG LMVATKYEVS VYALKDTLTS RPAQGVVTTL ENVSPPRRAR VTDATETTIT ISWRTKTETI TGFQVDAVPA NGQTPIQRTI KPDVRSYTIT GLQPGTDYKI YLYTLNDNAR SSPVVIDAST AIDAPSNLRF LATTPNSLLV SWQPPRARIT GYIIKYEKPG SPPREVVPRP RPGVTEATIT GLEPGTEYTI YVIALKNNQK SEPLIGRKKT DELPQLVTLP HPNLHGPEIL DVPST

Characteristics: Recombinant Human Fibronectin fragment/RetroNectin is produced with our E. coli expression system.The target protein is expressed with sequence (Pro1270-Ser1546&Ala1721-Thr2016) of Human Fibronectin.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Fibronectin

Background: "Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains, fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of 63 kDa containing a central cell-binding domain (type III repeat, 8, 9, 10), a high affinity heparin-binding domain II (type III repeat, 12, 13, 14), and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on RetroNectin. "
Synonyms: Fibronectin, Retronectin, CIG, FN, Cold-insoluble globulin

Molecular Weight: 62.7 kDa

UniProt: P02751