FKBP3 Protein (AA 1-224) (His tag)

Catalog No. ABIN2115464

Recombinant FKBP3 protein expressed in Escherichia coli (E. coli).

Quick Overview for FKBP3 Protein (AA 1-224) (His tag) (ABIN2115464)

Target: FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-224

Purification tag / Conjugate: This FKBP3 protein is labelled with His tag.

Purpose: Recombinant Human Peptidyl-Prolyl Cis-Trans Isomerase FKBP3 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID

Characteristics: Recombinant Human Peptidyl-prolyl cis-trans isomerase FKBP3 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp224) of Human FKBP3/FKBP25 fused with a 6His tag at the N-terminus..

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,1 mM DTT,10 % glycerol .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))

Alternative Name: Peptidyl-Prolyl Cis-Trans Isomerase FKBP3

Background: FKBP3 contains 1 PPIase FKBP-type domain, belongs to the FKBP-type PPIase family. FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. FKBP3 is a cis-trans prolyl isomerase enzyme that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin.
Synonyms: Peptidyl-prolyl cis-trans isomerase FKBP3,PPIase FKBP3,25 kDa FK506-binding protein,25 kDa FKBP,FKBP-25,FK506-binding protein 3,FKBP-3,Immunophilin FKBP25,Rapamycin-selective 25 kDa immunophilin,Rotamase,FKBP25

Molecular Weight: 27.3 kDa

UniProt: Q00688