Host-Nuclease Inhibitor Protein Gam (AA 1-138) Protein

Catalog No. ABIN2115469

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for Host-Nuclease Inhibitor Protein Gam (AA 1-138) Protein (ABIN2115469)

Target: Host-Nuclease Inhibitor Protein Gam

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-138

Purpose: Recombinant Mouse Sonic Hedgehog/SHH (C25II)

Sequence: MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Characteristics: Recombinant Bacteriophage Host-nuclease inhibitor protein gam is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val138) of Bacteriophage GAM fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Host-Nuclease Inhibitor Protein Gam

Background: Host-nuclease inhibitor protein gam(gam) comes from Enterobacteria phage lambda. Originally the gam gene product was assigned to the sequence shown and it was suggested that the gene kil protein is encoded from within this gene and corresponds to residues 41-138. For an alternative assignment of this gene see the entry for the kil gene protein of lambda. The function of the protein inhibits the host recBC-coded nuclease and thus promotes the late, rolling-circle mode of lambda DNA replication.
Synonyms: Host-nuclease inhibitor protein gam,gamma

Molecular Weight: 19.8 kDa

UniProt: Q62226