Cathepsin G Protein (CTSG)
(1 validation)Quick Overview for Cathepsin G Protein (CTSG) (ABIN2128355)
Target
See all Cathepsin G (CTSG) ProteinsProtein Type
Origin
Source
Purity
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Cathepsin G (CTSG) (AA 1-255) protein (GST tag)
CTSG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cathepsin G (CTSG) (AA 146-255) protein (GST tag)CTSG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cathepsin G (CTSG) (AA 21-261) protein (His-SUMO Tag)CTSG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Cathepsin G (CTSG) proteinCTSG Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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Restrictions
- For Research Use only
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- by
- NanoTemper Technologies
- No.
- #102720
- Date
- 11/05/2018
- Antigen
- CTSG
- Lot Number
- A14041801
- Method validated
- Unfolding Profile
- Positive Control
- ABIN2128355
- Negative Control
- Notes
Passed. ABIN2128355 showed a Ti of 63.8 °C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.
- Primary Antibody
- Secondary Antibody
- Full Protocol
- Dilute ABIN2128355 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 3.5µM.
- Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
- Run Tycho measurement.
- Experimental Notes
Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.
Validation #102720 (Unfolding Profile)!['Independent Validation' Badge]( "Successfully validated")
!['Independent Validation' Badge]( "Successfully validated")
Validation Images![Unfolding profile of ABIN2128355. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 63.8°C.]()
Unfolding profile of ABIN2128355. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 63.8°C.
Full Methods -
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Format
- Liquid
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Concentration
- 250 μg/mL
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Buffer
- Liquid 50 mM sodium acetate, pH 5.5, 0.8 M sodium chloride
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Storage
- 4 °C/-20 °C/-80 °C
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- Cathepsin G (CTSG)
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Alternative Name
- Cathepsin G
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Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
Target
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