CD40 Protein (CD40) (full length)

Catalog No. ABIN935307

This Recombinant CD40 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CD40 Protein (CD40) (full length) (ABIN935307)

Target: CD40

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Protein Characteristics: full length

Sequence: MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

Characteristics: Purified recombinant Mouse CD40 protein
Expression System: E.coli
Bioactivity: Determined by its ability to induce TNF-alpha and MIP-1 alpha production by Mouse splenocytes. The expected ED50 for this effect is

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Sterile filtered through a 0.2 μm filter. Lyophilized from 10 mM Na3PO4, pH 7.5 with 0.1 M arginine. Restore in water to a concentration of 0.1 - 1.0 mg/mL.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details

Target: CD40

Alternative Name: CD40

Background: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to immunodeficiency with hyper-IgM, a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L.
Alternative Names: CD-40, gp39 protein, soluble CD40 Ligand protein, CD 40, CD40, CD 40 protein, TNFSF5 protein, TRAP protein, CD-40 protein, CD154 protein, T-BAM protein

Molecular Weight: 16.4 kDa

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints