PPP1CA Protein

Catalog No. ABIN2131264

Recombinant PPP1CA protein expressed in Escherichia coli (E. coli).

Quick Overview for PPP1CA Protein (ABIN2131264)

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 80 % pure

Product Details

Application Details

Application Notes: PPP1CA protein (His tag) has been used in SDS PAGE, and may be suitable for use in other assays, to be determined by the end user.

Comment:

Biological activity: Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. 50 mM Tris-HCl buffer ( pH 8.0) containing 40 % glycerol, 0.15M NaCl, 1 mM DTT, 0.1 mM PMSF, 1 mM MnCl2

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C/-80 °C

Target Details

Target: PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))

Alternative Name: PPP1CA

Molecular Weight: 39.7 kDa (350aa), confirmed by MALDI-TOF

Pathways: M Phase, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Lipid Metabolism