WFDC2 Protein (AA 31-124) (His tag)

Catalog No. ABIN2181198

Recombinant WFDC2 protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for WFDC2 Protein (AA 31-124) (His tag) (ABIN2181198)

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 31-124

Purification tag / Conjugate: This WFDC2 protein is labelled with His tag.

Sequence: AA 31-124

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 10.9 kDa. The protein migrates as 19 kDa and 22-26 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Yang, Lu, Guo, Fellman, Ning, Lu, Menon, Kobayashi, Hanash, Celestino, Skates, Bast: "Human epididymis protein 4 antigen-autoantibody complexes complement cancer antigen 125 for detecting early-stage ovarian cancer." in: Cancer, (2019) (PubMed).

Target Details

Target: WFDC2 (WAP Four-Disulfide Core Domain 2 (WFDC2))

Alternative Name: HE4

Background: Human Epididymis Protein 4 (HE4) is also known as WAP four-disulfide core domain protein 2 (WFDC2), Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, is a member of the WFDC domain family. HE4 / WFDC2 is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is also a small secretory protein, which may be involved in sperm maturation.

Molecular Weight: 10.9 kDa