IGF1R Protein (AA 31-932) (His tag)

Catalog No. ABIN2181260

Product Details

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 31-932

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IGF1R protein is labelled with His tag.

Sequence: AA 31-932

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 104 kDa (single chain), 81 kDa (α subunit) and 23 kDa (β subunit). The protein migrates as 120 kDa, 81 kDa and 35 kDa under reducing (R) condition (SDS-PAGE).

Purity: >90 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Biological Activity Comment: Biological Activity: Measured by its ability to bind human IGF-I in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Target Details

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF-I R (IGF1R Products)

Background: The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60 % homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

Molecular Weight: 81.0 kDa, 23.8 kDa

NCBI Accession: NP_000866

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy