IGF1R Protein (AA 31-932) (His-Avi Tag)

Catalog No. ABIN7274862

Product Details

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Protein Type: Recombinant

Protein Characteristics: AA 31-932

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IGF1R protein is labelled with His-Avi Tag.

Purpose: Human IGF1R/CD221 Protein

Sequence: Glu31-Asn932

Characteristics: Recombinant Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Asn932.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IGF1R, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 54.1ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF1R (IGF1R Products)

Synonyms: CMT2N Protein, CD221 Protein, IGFIR Protein, IGFR Protein, JTK13 Protein, IGFIRC Protein, IGFR1 Protein, IGF-1R Protein, IGF1R Protein, DKFZp469I0618 Protein, igf-1r Protein, xIGF-1R Protein, xIGFR Protein, xigf1r Protein, A330103N21Rik Protein, D930020L01 Protein, hyft Protein, igf-ir Protein, IGF-IR Protein, IGF-IRb Protein, wu:fb03h06 Protein, IGF-IRa Protein, sb:eu1090 Protein, wu:fi20b10 Protein, alanyl-tRNA synthetase Protein, insulin like growth factor 1 receptor Protein, insulin-like growth factor 1 receptor Protein, insulin-like growth factor 1 Protein, insulin-like growth factor I receptor Protein, insulin like growth factor 1 receptor S homeolog Protein, insulin-like growth factor 1b receptor Protein, insulin-like growth factor 1a receptor Protein, AARS Protein, IGF1R Protein, Igf1r Protein, igf1r Protein, Igf1 Protein, LOC780503 Protein, LOC100136427 Protein, igf1r.S Protein, igf1rb Protein, igf1ra Protein

Background: The type 1 IGF receptor (IGF1R) is a transmembrane tyrosine kinase that is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target.

Molecular Weight: 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa(alpha subunit) and 52-55 kDa(beta subunit) based on Tris-Bis PAGE result.

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy