SPAM1 Protein (AA 36-482) (His tag)

Catalog No. ABIN2181785

Product Details

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 36-482

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SPAM1 protein is labelled with His tag.

Sequence: AA 36-482

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 52.1 kDa. The protein migrates as 64-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 °C in a 2.0 mL reaction mixture)

Biological Activity Comment: The activity of Human Hyaluronidase Protein, His Tag is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >60,000 U/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM NaCl, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Target Details

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Alternative Name: PH20 (SPAM1 Products)

Background: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N - acetyl - beta - D - glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Molecular Weight: 53.0 kDa

NCBI Accession: NP_694859