SPAM1 Protein
-
- Target See all SPAM1 Proteins
- SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purpose
- Human PH20 / SPAM1 Protein, Tag Free (MALS verified)
- Sequence
- Leu 36 - Ser 490
- Characteristics
- Human PH20, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Grade
- MALS verified
- Top Product
- Discover our top product SPAM1 Protein
-
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 346-445) protein (GST tag)
SPAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 1-509) protein (GST tag)SPAM1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 36-490) protein (His tag)Crystallography grade SPAM1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SPAM1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1) (AA 36-482) protein (His tag)SPAM1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
-
- Application Notes
- This protein carries no "tag". The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition due to glycosylation.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- 50 mM Tris, 100 mM NaCl, pH 7.5
- Storage
- -20 °C
- Storage Comment
- -20°C
-
- Target
- SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))
- Alternative Name
- / SPAM1 (SPAM1 Products)
- Synonyms
- AV039194 Protein, Ph-20 Protein, PH-20 Protein, PH20 Protein, HYA1 Protein, HYAL1 Protein, HYAL3 Protein, HYAL5 Protein, SPAG15 Protein, Spam Protein, XEH1 Protein, spam1 Protein, im:6902904 Protein, zgc:158668 Protein, sperm adhesion molecule 1 Protein, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) L homeolog Protein, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) Protein, Spam1 Protein, SPAM1 Protein, spam1.L Protein, spam1 Protein
- Background
-
Synonyms: SPAM1,PH-20,HYAL3,HYA1,HYAL1,HYAL5,SPAG15,
Description: Hyaluronidase PH-20 is also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N - acetyl - beta - D - glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. - Molecular Weight
- 52.6 kDa
- NCBI Accession
- NP_694859
-
