FcRn Protein (AA 24-297) (His tag,Strep Tag,Biotin)

Catalog No. ABIN2444126

Product Details

Target: FcRn (neonatal Fc Receptor (FcRn))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 24-297

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FcRn protein is labelled with His tag,Strep Tag,Biotin.

Brand: MABSol®,UltraLys

Sequence: AA 24-297

Specificity: The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Characteristics: The subunit FCGRT carries a polyhistidine tag at the C-terminus. The subunit B2M carries a Strep II tag at the C-terminus. The protein has a calculated MW of 31.2 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 33 kDa (FCGRT), 13 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Target Details

Target: FcRn (neonatal Fc Receptor (FcRn))

Alternative Name: FcRn (FcRn Products)

Background: FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Molecular Weight: 31.2 kDa (FCGRT), 13.1 kDa (B2M)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process