FcRn Protein (AA 24-297) (His tag)

Catalog No. ABIN7274675

Recombinant FcRn protein expressed in HEK-293 Cells.

Quick Overview for FcRn Protein (AA 24-297) (His tag) (ABIN7274675)

Target: FcRn (neonatal Fc Receptor (FcRn))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-297

Purification tag / Conjugate: This FcRn protein is labelled with His tag.

Purpose: Cynomolgus/Rhesus macaque FcRn Protein

Sequence: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

Specificity: Uni-Prot: Q8SPV9 (FCGRT), Q8SPW0 (B2M)

Characteristics: Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-Terminus of FCGRT.It contains Ala24-Ser297 (FCGRT) and Ile21-Met119 (B2M).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus/Rhesus macaque FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 uM as determined in SPR assay (Biacore T200).,Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind Cynomolgus/Rhesus macaque FcRn, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test).,Cynomolgus/Rhesus macaque FcRn on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus/Rhesus macaque FcRn is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in 20 mM PB, 250 mM NaC ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FcRn (neonatal Fc Receptor (FcRn))

Alternative Name: FcRn

Background: The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Molecular Weight: 31.5 kDa (FCGRT) and 11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT) and 13-15 kDa (B2M) based on Bis-Tris PAGE result.

UniProt: Q8SPV9

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process