FcRn Protein (AA 22-297) (His tag,Strep Tag)

Catalog No. ABIN2870741

This Recombinant FcRn protein is produced in HEK-293 Cells. There are 2 publications available.

Quick Overview for FcRn Protein (AA 22-297) (His tag,Strep Tag) (ABIN2870741)

Target: FcRn (neonatal Fc Receptor (FcRn))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 22-297

Purification tag / Conjugate: This FcRn protein is labelled with His tag,Strep Tag.

Brand: MABSol®

Sequence: AA 22-297

Characteristics: Mouse FcRn / FCGRT & B2M Heterodimer Protein, produced by co-expression of FCGRT and B2M, has a calculated MW of 33 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with his-tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The predicted N-terminus is Ser 22(FCGRT) & Ile 21(B2M). The reducing (R) protein migrates as 45-50 kDa (FCGRT) and 18 kDa (B2M) respectively due to glycosylation.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

References

Yang, Kim, Seong, Tae, Kwon: "Comparative studies of the serum half-life extension of a protein via site-specific conjugation to a species-matched or -mismatched albumin." in: Biomaterials science, Vol. 6, Issue 8, pp. 2092-2100, (2018) (PubMed).

Kenniston, Taylor, Conley, Cosic, Kopacz, Lindberg, Comeau, Atkins, Bullen, TenHoor, Adelman, Sexton, Edwards, Nixon: "Structural basis for pH-insensitive inhibition of immunoglobulin G recycling by an anti-neonatal Fc receptor antibody." in: The Journal of biological chemistry, Vol. 292, Issue 42, pp. 17449-17460, (2017) (PubMed).

Target Details

Target: FcRn (neonatal Fc Receptor (FcRn))

Alternative Name: FcRn

Background: FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Molecular Weight: 32.9 kDa (FCGRT) & 14.7 kDa (B2M)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process