RNASEH Protein

Catalog No. ABIN3200996

This Recombinant RNASEH protein is expressed in Escherichia coli (E. coli) and has been mentioned in 3+ publications.

Quick Overview for RNASEH Protein (ABIN3200996)

Target: RNASEH (Ribonuclease H (RNASEH))

Protein Type: Recombinant

Biological Activity: Active

Origin: E. coli

Source: Escherichia coli (E. coli)

Purity: > 95 % protein determined by SDS-PAGE (CBB staining)

Product Details

Characteristics: Specific Activity: 100, 000 units/mg protein
Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37 C.

Application Details

Application Notes: 1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA.
2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT)
3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 50 units/L

Buffer: 50 units/ul in 20 mM Tris-HCl ( pH 7.5), 100 mM KCl, 1 mM DTT, 50 % Glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Gubler: "Second-strand cDNA synthesis: mRNA fragments as primers." in: Methods in enzymology, Vol. 152, pp. 330-5, (1987) (PubMed).

Donis-Keller: "Site specific enzymatic cleavage of RNA." in: Nucleic acids research, Vol. 7, Issue 1, pp. 179-92, (1979) (PubMed).

Vournakis, Efstratiadis, Kafatos: "Electrophoretic patterns of deadenylylated chorion and globin mRNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 8, pp. 2959-63, (1976) (PubMed).

Target Details

Target: RNASEH (Ribonuclease H (RNASEH))

Background: Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.

UniProt: P0A7Y4