SSB Protein

Catalog No. ABIN2452175

This Native SSB protein is produced in Escherichia coli (E. coli). There are 2 publications available.

Quick Overview for SSB Protein (ABIN2452175)

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Protein Type: Native

Biological Activity: Active

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 95 % purity as determined by SDS-PAGE (CBB staining)

Product Details

Application Details

Application Notes: 1. Functional single-stranded DNA-binding protein for studying DNA replication and recombination
2. Enhancement of the specificity and yield of PCR

Comment:

The absence of endonucleases and exonucleases was confirmed.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5 mg/mL

Buffer: 20 mM Tris-HCl ( pH 7.6), 200 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50 % glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Krauss, Sindermann, Schomburg, Maass: "Escherichia coli single-strand deoxyribonucleic acid binding protein: stability, specificity, and kinetics of complexes with oligonucleotides and deoxyribonucleic acid." in: Biochemistry, Vol. 20, Issue 18, pp. 5346-52, (1982) (PubMed).

Weiner, Bertsch, Kornberg: "The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication." in: The Journal of biological chemistry, Vol. 250, Issue 6, pp. 1972-80, (1975) (PubMed).

Target Details

Target: SSB (Sjogren Syndrome Antigen B (SSB))

Background: E.coli single-stranded DNA binding protein (SSB) binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination in vivo. The SSB gene was expressed as the recombinant protein in E.coli highly purified. The molecular mass is 18.9 kDa.

UniProt: P0AGE0