RecA (Active) Protein

Catalog No. ABIN2452179

This Recombinant protein is expressed in Escherichia coli (E. coli) and has been mentioned in 2+ publications.

Quick Overview for RecA (Active) Protein (ABIN2452179)

Target: RecA

Protein Type: Recombinant

Biological Activity: Active

Origin: E. coli

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 90 % by SDS-PAGE (CBB staining)

Product Details

Characteristics: The product is over-expressed as a recombinant protein and highly purified by several steps of chromatography. A single band is observed by SDS-PAGE at 38 kD.

Application Details

Application Notes: 1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization (2).
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.6 mg/mL

Buffer: 20 mM Tris-HCl ( pH 8.0), 1 mM EDTA, 150 mM KCl, 1 mM DTT, 50 % glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20 C (For long term storage: -70 C)

References

Walker: "Understanding the complexity of an organism's responses to DNA damage." in: Cold Spring Harbor symposia on quantitative biology, Vol. 65, pp. 1-10, (2003) (PubMed).

Taidi-Laskowski, Tyan, Honigberg, Radding, Grumet: "Use of RecA protein to enrich for homologous genes in a genomic library." in: Nucleic acids research, Vol. 16, Issue 16, pp. 8157-69, (1988) (PubMed).

Target Details

Target: RecA

Background: E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loop and Holliday structure in homologous recombination reaction, and coprotease activities that promote self-cleavages of LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its homologs in eukaryotes are Rad51 protein and Dmcl protein.

UniProt: P0A7G6