HIV-1 p17 Protein

Catalog No. ABIN2452193

This Recombinant HIV-1 p17 protein is produced in Escherichia coli (E. coli). There are 3 publications available.

Quick Overview for HIV-1 p17 Protein (ABIN2452193)

Target: HIV-1 p17 (Human Immunodeficiency Virus 1 Matrix (HIV-1 p17))

Protein Type: Recombinant

Origin: Human Immunodeficiency Virus (HIV)

Source: Escherichia coli (E. coli)

Application: Dot Blot (DB), SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Purity: > 90 % by SDS-PAGE (CBB staining)

Product Details

Application Details

Application Notes: 1) It can be used as p17 antigen in detection of anti-HIV-1 p17 antibody in Western blotting or ELISA.
2) It can be used as a standard for quantitative analysis of HIV-1 p17 antigen.
3) It can be used in studies of structure and function of AIDS virus as a matrix protein that constitutes HIV-1 core.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.23 mg/mL

Buffer: 50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol

Storage: -20 °C

References

Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).

Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

Target Details

Target: HIV-1 p17 (Human Immunodeficiency Virus 1 Matrix (HIV-1 p17))

Alternative Name: HIV-1 Gag p17

Target Type: Viral Protein

Background: HIV-1 Gag p17 is the matrix protein of AIDS virus, HIV-1, and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable for reproduction of AIDS virus and is essential component of AIDS virus particles. This protein was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p17 coding region of HIV-1 virus, subtype B, and was highly purified by several steps of chromatography. Its molecular weight is 17 kD, same as that of p17 purified from AIDS virus particles.