HIV1RT Protein

Catalog No. ABIN2452197

This Recombinant HIV1RT protein is expressed in Escherichia coli (E. coli) and has been mentioned in 3+ publications.

Quick Overview for HIV1RT Protein (ABIN2452197)

Target: HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human Immunodeficiency Virus (HIV)

Source: Escherichia coli (E. coli)

Application: Dot Blot (DB), Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Purity: > 90 % by SDS-PAGE (CBB staining)

Product Details

Characteristics: Activity: 5,000 units/mL

Application Details

Application Notes: 1) It is extremely effective for screening new specific inhibitors for HIV virus as a drug for treating AIDS.
2) Generally,Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However,by using this enzyme in combination as an antigen,the detection will be more sensitive.
3) Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.
4) Standards for SDS-PAGE

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 50 % glycerol, 40 mM Tris-HCl ( pH 8.3), 50 mM NaCl, 5 mM MgCl2, 0.1 % Triton X-100, 1 mM DTT

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Saitoh, Iwasaki, Nakata, Adachi, Shinagawa: "Overproduction of human immunodeficiency virus type I reverse transcriptase in Escherichia coli and purification of the enzyme." in: Microbiology and immunology, Vol. 34, Issue 6, pp. 509-21, (1990) (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

Holtermüller, Bohlen, Castro, Weis: "[Considerations on antacid therapy]." in: Medizinische Klinik, Vol. 72, Issue 30, pp. 1229-41, (1977) (PubMed).

Target Details

Target: HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))

Alternative Name: HIV-1 Reverse Transcriptase

Target Type: Viral Protein

Background: HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase derived from HIV-1 (AIDS virus), subtype B origin. It also has RNaseH activity and is an enzyme indispensable for reproduction of AIDS virus. This protein is uniquely over-expressed as a recombinant protein in E. coli by a patented method and highly purified. It is composed of two subunits whose molecular weights are 66 kD and 51 kD, same as the those of the enzyme purified from AIDS virus particles.