Taq SSB (Active) Protein

Catalog No. ABIN2452212

This Recombinant protein is expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for Taq SSB (Active) Protein (ABIN2452212)

Target: Taq SSB

Protein Type: Recombinant

Biological Activity: Active

Origin: Thermus aquaticus

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func)

Purity: > 95 % of protein determined by SDS-PAGE (CBB staining)

Product Details

Characteristics: The absence of endonucleases and exonucleases was confirmed.

Application Details

Application Notes: Stabilizes single-stranded DNA in DNA replication,repair,and recombination

Comment:

Activity: Single-stranded DNA binding activity was confirmed.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 50 mM Tris-HCl ( pH 8. 0), 200 mM NaCl, 0. 1 mM dithiothreitol, 0. 5 mM EDTA, 50 % glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Dabrowski, Olszewski, Piatek, Kur: "Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification." in: Protein expression and purification, Vol. 26, Issue 1, pp. 131-8, (2002) (PubMed).

Target Details

Target: Taq SSB

Background: Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA. It plays important roles in DNA replication and recombination. Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa, same as that of the natural protein.

UniProt: Q9KH06