GDF5 Protein

Catalog No. ABIN2468474

This Recombinant GDF5 protein is produced in Escherichia coli (E. coli).

Quick Overview for GDF5 Protein (ABIN2468474)

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: < 98 % by SDS-PAGE gel and HPLC analyses.

Product Details

Sequence: APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

Characteristics: Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Endotoxin Level: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: As with any protein, exposing GDF-5 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -20 °C

Storage Comment: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted GDF-5 stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Expiry Date: 24 months

Target Details

Target: GDF5 (Growth Differentiation Factor 5 (GDF5))

Alternative Name: GDF-5

Background: GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 348 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant murine GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids.

Gene ID: 8200

NCBI Accession: NP_000548

OMIM: 4503969

UniProt: P43026