FASL Protein (Soluble)
Quick Overview for FASL Protein (Soluble) (ABIN2469071)
Target
See all FASL ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- Soluble
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Sequence
- HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL
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Characteristics
- Biological activity was determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is < 10.0 ng/mL, corresponding to a specific activity of > 1 x 105 units/mg.
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Endotoxin Level
- Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
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Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (His tag)
custom-made FASL Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-279) protein (His tag)custom-made FASL Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)FASL Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (GST tag)FASL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Fas Ligand (TNF Superfamily, Member 6) (FASL) protein (Fc Tag)FASL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Fas Ligand (TNF Superfamily, Member 6) (FASL) proteinFASL Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Handling Advice
- As with any protein, exposing sFas Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Storage
- -20 °C
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Storage Comment
- The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted sFas Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
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Expiry Date
- 24 months
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- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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Alternative Name
- Fas Ligand
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Background
- Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand is a 17.9 kDa protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.
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Gene ID
- 356
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NCBI Accession
- NP_000630
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OMIM
- 4557329
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UniProt
- P48023
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Pathways
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Target
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