Protein L Protein (His tag)

Catalog No. ABIN2566879

This Recombinant Protein L protein is expressed in Escherichia coli (E. coli).

Quick Overview for Protein L Protein (His tag) (ABIN2566879)

Target: Protein L

Protein Type: Recombinant

Origin: Others

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: >98 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This Protein L protein is labelled with His tag.

Application Details

Application Notes: This recombinant protein can be used for WB. For research use only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -80 °C,-20 °C

Storage Comment: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: Protein L

Background: Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction, from which the name was suggested. Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). In humans and mice, kappa (k) light chains predominate. The remaining immunoglobulins have lambda (l) light chains. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).