Streptavidin Protein
Quick Overview for Streptavidin Protein (ABIN934746)
Target
See all Streptavidin ProteinsProtein Type
Origin
Source
Application
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Characteristics
- Purified homogeneous preparation of Streptavidin protein
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Streptavidin protein (HRP)
Origin: Streptomyces avidinii WB, ELISA, IHC, DB, IF
Streptavidin protein (PE)Origin: Streptomyces avidinii FACS, IM
Streptavidin (AA 37-159) protein (His tag)Origin: Streptomyces Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
Streptavidin protein (Rhodamine)Origin: Streptomyces avidinii
Streptavidin protein (Atto 425)Origin: Streptomyces avidinii
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Supplied as a lyophilized powder. Resonstitute with deionized water.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Aliquot and store at -20 °C.
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- Streptavidin
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Background
- Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin, the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ~10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton) and proteolytic enzymes. .
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Molecular Weight
- 55 kDa
Target
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