BMP4 Protein (AA 303-408)

Catalog No. ABIN2666445

Recombinant BMP4 protein expressed in Escherichia coli (E. coli).

Quick Overview for BMP4 Protein (AA 303-408) (ABIN2666445)

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purity: > 97 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Product Details

Protein Characteristics: AA 303-408

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: BMP-4 induces alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 5 - 15 ng/ml, corresponding to a specific activity of 0.67 - 2.0 x 105 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute 10 mM citric acid to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: BMP4 (Bone Morphogenetic Protein 4 (BMP4))

Alternative Name: BMP-4

Background: Bone morphogenetic proteins (BMPs) are multi-functional growth factors that belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Three type I receptors have been identified: type IA and IB BMP receptors and type IA activin receptors. Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. BMP-4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, rat, and zebrafish. BMP-2 and BMP-4 are highly similar, they have 92 % amino acid sequence identity. BMP-4 is required for mesoderm formation and patterning in mice, and BMP-4 KO mice are embryonic lethal. BMP-4 has an important role in postnatal tooth cytodifferentiation. Overexpression of BMP-4 has been associated with fibrodysplasia ossificans progressiva (FOP).

Molecular Weight: The 106 amino acid recombinant protein has a predicted molecular mass of approximately 12 kDa. Recombinant BMP-4 is a disulphide bond linked homodimer. The predicted N-terminal amino acid is Lys.

Pathways: Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development