MMP3 Protein (AA 18-477, His9, N-Term)

Catalog No. ABIN2666507

This Recombinant MMP3 protein is expressed in HEK-293 Cells.

Quick Overview for MMP3 Protein (AA 18-477, His9, N-Term) (ABIN2666507)

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 18-477, His9, N-Term

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 1.0 EU per μg of protein as determine by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Human MMP-3 cleaves the peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 with an activity above 135 pmol/min/μg.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening.

Buffer: 0.22 μm filtered protein solution is in TCN (25 mM TRIS, 5 mM CaCl2, 150 mM NaCl, 20 % Glycerol, pH 7.5).

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -70°C for six months.

Target Details

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Alternative Name: MMP-3

Background: MMP-3 is a member of matrix metalloproteinase family proteins (MMPs), members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins in normal physiological processes such as embryonic development, reproduction, and tissue remodeling as well as in disease processes such as arthritis and metastasis. MMP-3 consists of a prodomain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin-like repeats) domain. Substrates of MMP-3 include collagen α chains, aggrecan, laminin, fibronectin, elastin, casein, α1-antitrypsin, myelin basic protein, IL1β, IGFBP3, pro MMP1, pro MMP7, pro MMP8, pro MMP9, and pro MMP13. MMP-3 is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. It has also been known that a polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infraction due to lower MMP3 proteolytic activity and higher ECM deposition in atherosclerotic lesions.

Molecular Weight: Predicted molecular mass of approximately 54 kDa. The protein migrates at about 55 kDa in DTT-reducing conditions and about 60 kDa in non-reducing condition by SDS-PAGE. The N-terminal amino acid is Histidine.