Prolactin Protein (PRL) (AA 29-227, N-Term)

Catalog No. ABIN2666567

This Recombinant Prolactin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Prolactin Protein (PRL) (AA 29-227, N-Term) (ABIN2666567)

Target: Prolactin (PRL)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 29-227, N-Term

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.1 EU per μg of protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Human Prolactin induces proliferation of MCF7 breast cancer cells in a dose dependent manner. The ED50 is 60 - 350 ng/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: Prolactin (PRL)

Alternative Name: Prolactin

Background: Prolactin, also known as luteotropin, is a hormone that is secreted by the pituitary gland and is best known for its role in enabling female mammals to produce milk. However, prolactin has also been shown to play essential roles in the regulation of immune functions, pancreatic development, angiogenesis, reproduction, osmoregulation, and metabolism. Besides the pituitary origin, prolactin is found in other tissues and cells such as adipocytes, immune cells, skin, prostate, brain, and decidua. This hormone is produced in increasing amounts during pregnancy and suckling. It acts on the mammary gland by initiating and maintaining lactation in the postpartum period. Epidemiological studies have demonstrated that circulating high levels of prolactin increase the risk of breast cancer. Prolactin may promote mammary tumor initiation via the JAK2/STAT5 signaling pathway. Human prolactin has a 60 % amino acid sequence homology with its murine counterpart. In a knockout animal study, it was shown that female mice lacking the expression of prolactin were infertile, but does not prevent them from manifesting spontaneous maternal behaviors. In addition, these animals show no sign of alterations in myelopoiesis and lymphopoiesis, suggesting that prolactin may not play an indispensable role in lymphocyte development and myeloid differentiation.

Molecular Weight: The 200 amino acid recombinant protein has a predicted molecular mass of approximately 23 kDa. The protein migrates approximately at 25 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met.

Pathways: JAK-STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus