CCL20 Protein (AA 27-96)

Catalog No. ABIN2666608

This Recombinant CCL20 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL20 Protein (AA 27-96) (ABIN2666608)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Multiplex Assay (MA)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 27-96

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-hCCR6 transfectants in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CCL20

Background: CCL20 is an inflammatory cytokine involved in the recruitment of leukocytes to sites of injury and inflammation. CCL20 is expressed at a low basal level, but it is induced by proinflammatory cytokines and Toll-like receptor (TLR) agonists. During infections, CCL20 expression is stimulated in epithelial cells, and immature CCR6+ dendritic cells (DCs) are recruited. Thereafter, CCR6 is downregulated during DC maturation, and the DCs migrates to the lymph node under the expression of CCR7. CCL20 is the only ligand for CCR6. Nevertheless, mouse and human β -defensin-1 and -2, small cationic antimicrobial peptides, bind and activate CCR6. Contrarily, these β-defensins have lower affinities for CCR6 than CCL20. In fact, human CCL20 has potent antimicrobial activity.

Molecular Weight: The 70 amino acid recombinant protein has a predicted molecular mass of approximately 8.0 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein at approximately 12 kDa by SDS-PAGE. The N-terminal amino acid is Ala.

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection