CD70 Protein (AA 47-195)

Catalog No. ABIN2666756

This Recombinant CD70 protein is expressed in CHO Cells.

Quick Overview for CD70 Protein (AA 47-195) (ABIN2666756)

Target: CD70 (CD70 Molecule (CD70))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: CHO Cells

Application: Flow Cytometry (FACS)

Purity: > 90 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 47-195

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.1 EU per μg of protein as determine by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Mouse CD27L induces proliferation of mouse T cells in the presence of anti-mouse CD3. The ED50 is 1.4 - 5.6 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD27L

Background: CD27L is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. Mouse CD27L and its human counterpart share about 56 % amino acid sequence identity. The interaction between CD27L and CD27, a protein of the TNF receptor superfamily expressed on NK cells and subsets of T and B cells, provides costimulatory signals that are required for T cell proliferation, clonal expansion, and promotion of effector T cell formation. CD27L-CD27 interaction on mouse B cells on the other hand, has been shown to inhibit terminal differentiation of B cells into plasma cells. CD27L expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. CD27 interaction with recombinant soluble CD27L protein (sCD27L), has shown that sCD27L enhances IFN-γ secretion by CD56(bright) NK cells in the presence of IL-12, and these NK cells were able to suppress HCV replication. Also, CD8+ T cells stimulated with sCD27L in the presence of Ag showed enhanced proliferation and production of IL-2 and IFN-γ in vitro. In addition, administration of Ag and soluble CD27L induces expansion of Ag-specific CD8+ T cells in vivo. Data suggests that sCD27L is a potent stimulator of antiglioma immune responses that depend on CD8+ T cells, therefore, sCD27L might be an adjuvant for future immunotherapy trials for glioblastoma.

Molecular Weight: The 170 amino acid recombinant protein has a predicted molecular mass of approximately 18.6 kDa. The protein migrates at about 35 kDa in DTT-reducing conditions by SDS-PAGE. The N-terminal amino acid is His.

Pathways: Cancer Immune Checkpoints