CCL20 Protein (AA 26-96)

Catalog No. ABIN2666785

This Recombinant CCL20 protein is expressed in Escherichia coli (E. coli).

Quick Overview for CCL20 Protein (AA 26-96) (ABIN2666785)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 26-96

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-hCCR6 transfectants in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: CCL20

Background: Rat CCL20 was initially identified in ischemic brain tissue and called ST38. In experimental autoimmune panencephalomyelitis, CCL20 expression correlates with the onset of inflammation. In experimental autoimmune encephalomyelitis (EAE), CCL20 and CCR6 mRNAs are induced in the CNS of SJL mice, CCL20 is expressed mainly by CNS-infiltrating leukocytes, and it can be expressed by astrocytes as an additional source of CCL20 during disease relapses. CCL20 is involved in the recruitment of leukocytes to sites of injury and inflammation. CCL20 is constitutively expressed in liver, lung, and mucosal epithelial cells (small intestine and colon). CCL20 is expressed at a low basal level, but its expression is amplified by proinflammatory cytokines and toll-like receptor (TLR) agonists. During infections, CCL20 expression is stimulated in epithelial cells, and immature CCR6+ dendritc cells (DCs) are recruited. Thereafter, the CCR6 expression is downregulated during DC maturation and these migrate to the lymphoid node under the expression of CCR7. CCL20 is the only ligand for CCR6. Nevertheless, mouse and human β -defensin-1 and -2, small cationic antimicrobial peptides, bind and activate CCR6. Contrarily, these β-defensins have lower affinities for CCR6 than CCL20.

Molecular Weight: The 71 amino acid recombinant protein has a predicted molecular mass of approximately 8.2 kDa. The DTT-reduced protein migrates at approximately 8 kDa and non-reduced protein at approximately 10 kDa by SDS-PAGE. The N-terminal amino acid is Ala.

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection