Mesothelin Protein (MSLN) (AA 298-582, C-Term)

Catalog No. ABIN2666851

This Recombinant Mesothelin protein is produced in HEK-293 Cells.

Quick Overview for Mesothelin Protein (MSLN) (AA 298-582, C-Term) (ABIN2666851)

Target: Mesothelin (MSLN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: ELISA

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 298-582, C-Term

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Human CA125/MUC16 when immobilized at 2 μg/ml is able to bind recombinant Mesothelin in a dose dependent manner with a BD50 of 0.03 - 0.09 μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: Mesothelin (MSLN)

Alternative Name: Mesothelin

Background: Mesothelin (MSLN) was initially cloned from a library of human pancreatic cancer cells. It was also identified by a monoclonal antibody, MAb K1, which recognized a glycoprotein present on the surface of mesothelial cells. Mouse and human mesothelin are 69 % identical. Mouse MSLN gene encodes a ~71 kD precursor protein of 625 amino acids. The C-terminal glycosylphosphatidylinositol (GPI) anchor addition signal is removed and replaced with a GPI anchor. The MSLN precursor is cleaved by furin into two products, a ~31 kD mature megakaryocyte potentiating factor and a ~40 kD GPI-anchored membrane-bound mature mesothelin. The 40 kD GPI-anchored mesothelin can be released by treatment with phosphatidylinositol-specific phospholipase C. Soluble mesothelin specifically binds to ovarian carcinoma cell line OVCAR-3, mediated by the cancer antigen 125 (CA125), a mucin-like glycoprotein on the surface of tumor cells. CA125 and mesothelin are co-expressed in advanced grade ovarian adenocarcinoma, and it has been speculated that CA125 might contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to mesothelin. Also, mesothelin and CA125/mucin 16 (CA125/MUC16) are co-overexpressed at the invading edges of pancreatic cancer tissues, and their expression correlates with poor survival rates. Mesothelin expression in the mouse embryo is high on days 7 and 17 of gestation, but absent on day 11 and low on day 15, suggesting developmental regulation. Nevertheless, mesothelin function is not essential for growth or reproduction in mice.

Molecular Weight: The 291 amino acid recombinant protein has a predicted molecular mass of approximately 32.9 kDa. The DTT-reduced and non-reduced protein migrate at approximately at 50-55 kDa by SDS-PAGE. The N-terminal amino acid is Glu.

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process