CCL24 Protein (AA 27-119)

Catalog No. ABIN2666899

This Recombinant CCL24 protein is produced in Escherichia coli (E. coli).

Quick Overview for CCL24 Protein (AA 27-119) (ABIN2666899)

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 27-119

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Bioactivity was measured by its property to chemoattract BaF3-mCCR3 transfectants in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: CCL24

Background: CLL24 was initially cloned from a library of activated macrophages, and using human eosinophils, it was shown that it binds to the receptor of eotaxin-1 (CCL11) and MCP-4 (CCL13). In addition, CCL24 shares the CCR3 receptor with CCL26, CCL5, and CCL7. Eotaxin/CCR3 expression has been studied extensively in the pathogenesis of asthma and allergy. In addition, the eotaxin/CCR3 axis has been associated to additional inflammatory and autoimmune disorders including inflammatory bowel disease, multiple sclerosis, eosinophilic esophagitis, and rheumatoid arthritis. CCL24 has also been associated to neovascular age related macular degeneration.

Molecular Weight: The 93 amino acid recombinant protein has a predicted molecular mass of approximately 10.4 kDa. The DTT-reduced protein migrates at approximately 12 kDa and non-reduced protein migrates at 14 kDa by SDS-PAGE. The N-terminal amino acid is Valine.

Pathways: Regulation of Actin Filament Polymerization