SIGLEC5 Protein (AA 17-434, C-Term) (Fc Tag)

Catalog No. ABIN2666956

This Recombinant SIGLEC5 protein is produced in CHO Cells.

Quick Overview for SIGLEC5 Protein (AA 17-434, C-Term) (Fc Tag) (ABIN2666956)

Target: SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 17-434, C-Term

Purification tag / Conjugate: This SIGLEC5 protein is labelled with Fc Tag.

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 1.0 EU per μg of protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Recombinant human Siglec 5 is able to agglutinate human red blood cells with an ED50 of 0.625 - 2.5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA. After dilution, the cytokine can be stored at 4 °C for one month or from -20 °C to -70 °C for up to three months.

Concentration: 100 μg/mL

Buffer: 0.22 μm filtered protein solution is in 20 mM Tris, 150 mM NaCl, and pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))

Alternative Name: Siglec 5

Background: Siglec proteins (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region which contains a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. In the cytosolic domain, most siglecs contain a combination of tyrosine motifs that include immunoreceptor tyrosine-based inhibitory motifs (ITIMs) along with ITIM-like, Grb2-binding, and Fyn kinase sites. Siglecs are widely expressed on hematopoietic cells, often in a cell type specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell surface glycoproteins and glycolipids. Studies suggest that Siglecs may participate in cell-to-cell interactions or can act as receptors during the entry of viral or bacterial pathogens. In addition, the presence of ITIMs indicate that these molecules play a role in the suppression of immunoreceptor signaling. Siglecs can be classified into two subgroups with Siglec 1, 2, and 4 as one group and a Siglec 3/CD33-related subgroup (Siglec 3 and Siglec 5 - 14) as the second. Siglec 5, a human CD33-related Siglec, selectively regulates early recruitment of neutrophils in the lung during lipopolysaccharide-induced acute lung inflammation.

Molecular Weight: This 652 amino acid recombinant protein has a predicted molecular mass of approximately 72.7 kDa. The protein migrates at approximately 120 kDa in DTT-reduced conditions and approximately 250 kDa in non-reduced conditions by SDS-PAGE. The predicted N-term