CD40 Ligand Protein (CD40LG) (AA 114-261)

Catalog No. ABIN2667388

This Recombinant CD40 Ligand protein is produced in Escherichia coli (E. coli).

Quick Overview for CD40 Ligand Protein (CD40LG) (AA 114-261) (ABIN2667388)

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Product Details

Protein Characteristics: AA 114-261

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 5-10 ng/ml, corresponding to a specific activity of 1-2 x 105 units/mg, as determined by the dose-dependent stimulation of IL-8 production by human PBMCs. Results may vary with PBMC donors.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40L

Background: CD40L is a type II membrane protein that shares 78 % amino acid identity with its mouse counterpart. Human CD40L is a polypeptide of 261 amino acids, consisting of 22, 24, and 215 amino acids for the cytoplasmic, transmembrane, and extracellular domains respectively. Soluble forms of CD40L (sCD40L) have been identified, and a 18 kD fragment has the ability to form homotrimers and bind to CD40 to induce biological signals. The 18 kD protein has full activity in B cell proliferation and differentiation assays. Activated platelets express CD40L on their surface and constitute the major source of sCD40L (>95 % in blood). Platelet-derived CD40L regulates neutrophil recruitment and lung damage in sepsis, and it has been suggested that MMP-9 regulates CD40L shedding in abdominal sepsis. Platelet-associated CD40L has been linked to progression of atherosclerotic disease, and elevated plasma levels of sCD40L in patients seems to be a reliable predictor of cardiovascular events, such as atherosclerotic plaque rupture and acute coronary syndromes. Also, platelet-associated CD40L expression is increased in idiopathic thrombocytopenic purpura patients, and it has been speculated that platelet-associated CD40L drives the activation of autoreactive B lymphocytes in this disease. In addition, high plasma levels of sCD40L have been connected to HIV-associated neurocognitive disorders (HAND).

Molecular Weight: The 149 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 16.3 kDa. The predicted N-terminal amino acid is Met.

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints