FGF9 Protein (AA 3-208)

Catalog No. ABIN2667403

This Recombinant FGF9 protein is produced in Escherichia coli (E. coli).

Quick Overview for FGF9 Protein (AA 3-208) (ABIN2667403)

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >95 % , as determined by Coomassie stained SDS-PAGE gel and HLPC analysis.

Product Details

Protein Characteristics: AA 3-208

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0 x 106 units/mg, as measured by its ability to stimulate thymidine uptake by BaF3 cells expressing FGF receptors in a dose dependent manner.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Alternative Name: FGF-9

Background: FGF-9 is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family. FGF-9 was first identified as a secretory protein from the human glioma cell line NMC-G1 and is reported to be widely expressed in rat brain. In the central nervous system, FGF-9 acts as an autocrine and a paracrine growth factor that is required for the development, growth, and survival of neuronal cells. During prenatal stages, FGF-9 expression is crucial for normal lung and airway development. FGF-9 null mutant mice show the phenotype of severe lung hypoplasia, which results in perinatal death. FGF-9 is key in stimulating cell proliferation after arterial, skeletal, and hepatic injuries. FGF-9 is also involved in tumorgenesis, enhancing transformation and progression of several malignant diseases, including prostate, lung, and colon cancer.

Molecular Weight: The 206 amino acid recombinant protein has a predicted molecular mass of approximately 23.2 kDa. The predicted N-terminal amino acid is Pro.