Interferon gamma Protein (IFNG) (AA 23-156)

Catalog No. ABIN2667442

This Recombinant Interferon gamma protein is produced in Escherichia coli (E. coli).

Quick Overview for Interferon gamma Protein (IFNG) (AA 23-156) (ABIN2667442)

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 23-156

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Specific activity is 9.2 x 105 units/mg, as determined in a cytopathic effect inhibition assay using EMC virus on L929 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma

Background: IFN-γ is a key component of the immune response to viral infections. It is produced by NK, NKT, and γ/δ T cells during early stages of infection and by CD4 and CD8 T cells later during the adaptive immune response. During infection, the role of IFN-γ includes protection, clearance, and modulation of the immune response. IFN-γ modulates epithelial cell functions and may play a role in intestinal wound healing as it might help preserve the integrity of the intestinal epithelium. It has been published that IFN-γ enhances IL-27 secretion, and the treatment of rats in a model of arthritis, IFN-γ/IL-27 successfully controlled arthritic inflammation and inhibited mediators of inflammation, angiogenesis, cell survival, apoptosis, and tissue damage. Nevertheless, IFN-γ has been associated with multiple inflammatory and autoimmune diseases such as arthritis, lupus nephritis, diabetic retinopathy, lung fibrosis, allergic conjunctivitis, and pediatric SLE. Rat IFN-γ protein has 39 % homology with humans and 85 % homology with mice proteins. Due to the high homology between rat and mouse IFN-γ, there is a strong affinity for rat IFN-γ on mouse cells.

Molecular Weight: The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.6 kDa. The DTT-reduced and non-reduced protein migrate at approximately 14 kDa by SDS-PAGE. The N-terminal amino acid is Met.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy